Puromycin aminonucleoside

CAS No. : 58-60-6

产品活性：Puromycin aminonucleoside (NSC 3056) 是一种氨基核苷类抗生素，为嘌呤霉素类似物。Puromycin aminonucleoside 诱导细胞凋亡 (apoptosis)。Puromycin aminonucleoside 可逆抑制二肽基肽酶 (dipeptidyl peptidase II) 和胞浆丙氨酸氨基肽酶。Puromycin aminonucleoside 诱导细胞迁移小体的分泌。

研究领域：Anti-infection | Apoptosis | Metabolic Enzyme/Protease

作用靶点：Bacterial | Apoptosis | Dipeptidyl Peptidase | Aminopeptidase | Antibiotic

In Vitro: Puromycin aminonucleoside (NSC 3056) (30 μg/mL) markedly increases p53 protein levels in podocytes. Puromycin aminonucleoside (NSC 3056)-induced podocyte apoptosis is p53 dependent. Puromycin aminonucleoside (NSC 3056) induces podocyte apoptosis in a time-dependent manner. The IC₅₀ values for PMAT-expressing and vector-transfected cells are 48.9 and 122.1 μM, respectively, suggesting expression of PMAT-enhanced cell sensitivity to Puromycin aminonucleoside. Puromycin aminonucleoside (NSC 3056) (250 μM) is toxic to both PMAT-expressing and vector-transfected cells. Puromycin aminonucleoside (NSC 3056) uptake in PMAT-expressing cells is fourfold higher at pH 6.6 than that at pH 7.4.

In Vivo:

Induction of Nephrotic syndrome (NS) model

Background

Puromycin aminonucleoside (PAN) induces cellular oxidant injury and increases in proteinuria, albumin (ALB), and creatinine clearance (Ccr) levels in the kidneys.

Specific Mmodeling Methods

Mice: Sprague Dawley rats •male •? 180-190 g
Administration: 50 mg/kg • iv • single dose

Note

Modeling Record

Molecular changes: Induced increases in proteinuria, albumin (ALB), and Ccr levels.

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