Thrombin from bovine plasma 凝血酶

Synonym:

Factor IIa;Thrombin;凝血酶

Product Description

Catalog Number:T4648

Storage Temperature: –20℃

CAS RN:9002-04-4

EC 3.4.21.5

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

The predominant form of thrombin in vivo is the zymogen, prothrombin (factor II), which is produced in the liver. The concentration of prothrombin in normal human plasma is 5–10 mg/dL.3 Prothrombin is a glycoprotein with a glycan content of 12%.

Prothrombin is cleaved in vivo by activated factor X, releasing the activation peptide and cleaving thrombin into light and heavy chains yielding catalytically active -thrombin. -Thrombin is composed of a light chain (A chain, MW 6 kDA) and a heavy chain (B chain,

MW 31 kDa). These two chains are joined by one disulfide bond.4 The B chain of human thrombin consists of a peptide portion (MW 29,485 Da) and a carbohydrate portion (MW 2,334 Da) with N-linked glycosylation at three Asn residues.5,6 Bovine thrombin contains 1.7% glucosamine, 1.8% sialic acid, 0.61% galactose, and 0.95% mannose.  

Thrombin also contains -carboxyglutamyl residues. These modified glutamyl residues are the result of carboxylation by a microsomal enzyme, vitamin Kdependent carboxylase. -Carboxyglutamyl residues are necessary for the Ca2+-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.4 Prothrombin is activated in vivo on the surface of a phospholipid membrane that binds the amino terminus of prothrombin along with factors Va and Xa. The activation process starts slowly because factor V is activated to factor Va by the initial, small amount of thrombin.

Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Preparation Instructions

The product is soluble in water (10 mg/ml), yielding a clear solution.

Storage/Stability

Stock solutions can be prepared at a concentration of 100 units/ml in a 0.1% (w/v) BSA solution. Stock solutions remain active for one week at 0–5℃. Solutions are most stable at pH 6.5, as a pH 7 will greatly reduce thrombin activity. Since thrombin solutions adsorb to glass, it is recommended to aliquot the solutions in plastic tubes and store at –20℃ for long-term storage. Store the lyophilized powder at –20℃. The product retains activity for at least 6 years.

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